14-3-3 beta/zeta, Antibody

14-3-3 protein zeta/delta; 1433Z; 143Z; Factor activating exoenzyme S; FAS; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ

For Research Use Only. Not for use in diagnostic procedures.

14-3-3 beta/zeta (Phospho-Ser184/186) antibody detects endogenous levels of 14-3-3 zeta/beta only when phosphorylated at serine 184 or serine 186.

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year.

Small volumes of anti-14-3-3 beta/zeta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000
ELISA: 1:5000

Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using 14-3-3 beta/zeta (Phospho-Ser184/186) antibody. 14-3-3 beta/zeta (Phospho-Ser184/186) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.

19,333 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta

14-3-3 protein zeta/delta

14-3-3 protein zeta/delta

KCIP-1  [Similar Products]

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Zupan L.A., J. Biol. Chem. 267:8707-8710(1992). Seluja G.A., Biochim. Biophys. Acta 1395:281-287(1998). The MGC Project Team; Genome Res. 14:2121-2127(2004).

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