A-kinase anchor protein 8 (AKAP8), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AKAP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7205936 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A-kinase anchor protein 8 (AKAP8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKAP8. The ELISA analytical biochemical technique of the MBS7205936 kit is based on AKAP8 antibody-AKAP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKAP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKAP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

76,108 Da

A kinase (PRKA) anchor protein 8

A-kinase anchor protein 8; A-kinase anchor protein, 95kDa

A-kinase anchor protein 8

AKAP95; AKAP-8; AKAP 95  [Similar Products]

AKAP8_HUMAN

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family.

Protein type: Mitochondrial; Adaptor/scaffold; Cell cycle regulation; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; mitochondrion; membrane; condensed chromosome; female pronucleus; nucleus

Molecular Function: zinc ion binding; double-stranded DNA binding

Biological Process: mitosis; mitotic chromosome condensation; signal transduction

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