Adenosine receptor A1, Polyclonal Antibody

Rabbit Anti-Human Adenosine receptor A1 Polyclonal; ADORA1 Antibody; Adenosine receptor A1 Antibody; Adenosine A1 Receptor Antibody; A1AR Antibody; A1R Antibody; AA1R Antibody; ADORA 1 antibody; RDC 7 Antibody; RDC7 Antibody; Ri Antibody; Adenosine receptor (A1) Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Detects ~37 kDa.

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C.

Small volumes of anti-ADORA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Immunology; Innate Immunity; Macrophage; Cardiovascular System; Atherosclerosis; Vascular Inflammation; Inflammatory Mediators; Cardiovascular System; Heart; Cancer; Apoptosis

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Adenosine receptor A1 Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~36.5 kDa Adenosine receptor A1 protein using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36.5 kDa.

13,348 Da

adenosine A1 receptor

adenosine receptor A1

Adenosine receptor A1

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

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