Full Product Name
LIPH siRNA (Mouse)
Product Synonym Names
Lipase member H
Product Gene Name
LIPH sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CIV3
Specificity
LIPH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LIPH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LIPH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LIPH sirna
siRNA to inhibit LIPH expression using RNA interference
Applications Tested/Suitable for LIPH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LIPH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001077363.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001083894.1
[Other Products]
UniProt Primary Accession #
Q8CIV3
[Other Products]
UniProt Secondary Accession #
Q3TRT3; Q3TTZ0; Q3UWA2; Q8BXB5; Q8CI45[Other Products]
UniProt Related Accession #
Q8CIV3[Other Products]
Molecular Weight
47,514 Da
NCBI Official Full Name
lipase member H isoform 1
NCBI Official Synonym Full Names
lipase, member H
NCBI Official Symbol
Liph [Similar Products]
NCBI Official Synonym Symbols
P3; H06; lpd1; lpd2; Lpdlr; PLA1B; mPA-PLA1; D16Wsu119e; C130037N08Rik
[Similar Products]
NCBI Protein Information
lipase member H
UniProt Protein Name
Lipase member H
UniProt Gene Name
Liph [Similar Products]
UniProt Entry Name
LIPH_MOUSE
UniProt Comments for LIPH
LIPH: Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG). Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7); also known as alopecia universalis congenita Mari type. A condition characterized by the presence of less than the normal amount of hair. Affected individuals have sparse or absent scalp, axillary and body hair and sparse eyebrows and eyelashes. Defects in LIPH are the cause of woolly hair autosomal recessive type 2 (ARWH2). A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrhexis nodosa and tapered ends. Some individuals may present with hypotrichosis. Belongs to the AB hydrolase superfamily. Lipase family.
Protein type: Secreted; EC 3.1.1.-; Phospholipase; Secreted, signal peptide
Cellular Component: extracellular space; membrane; plasma membrane; extracellular region
Molecular Function: sulfolactone hydrolase activity; retinyl-palmitate esterase activity; heparin binding; 3,4-dihydrocoumarin hydrolase activity; hydrolase activity; lipase activity; butyrolactone hydrolase activity; all-trans-retinyl-palmitate hydrolase activity; 1-oxa-2-oxocycloheptane lactonase activity; phospholipase activity; prenylcysteine methylesterase activity
Biological Process: lipid metabolic process; lipid catabolic process
Research Articles on LIPH
1. We finally isolate control and Enpp2-null dermal pipilla precursors and identify the expression and upregulation of LIPH, an alternative LPA-producing enzyme, suggesting that this gene could functionally compensate for the absence of Enpp2.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
