Full Product Name
ADO Antibody (C-term)
Product Synonym Names
2-aminoethanethiol dioxygenase; Cysteamine dioxygenase; ADO; C10orf22
Product Gene Name
anti-ADO antibody
[Similar Products]
Antibody/Peptide Pairs
ADO peptide (MBS9218115) is used for blocking the activity of ADO antibody (MBS9213973)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
206-233
3D Structure
ModBase 3D Structure for Q96SZ5
Species Reactivity
Human, mouse
Specificity
This ADO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-233 amino acids from the C-terminal region of human ADO.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ADO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADO antibody
Human thiol dioxygenases include cysteine dioxygenase
(CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase
(ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine,
whereas ADO adds 2 oxygen atoms to free cysteamine to form
hypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied by
OMIM].
Product Categories/Family for anti-ADO antibody
Metabolism; Signal Transduction
Applications Tested/Suitable for anti-ADO antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-ADO antibody
WB~~1:1000
Western Blot (WB) of anti-ADO antibody
ADO Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ADO antibody detected the ADO protein (arrow).
Flow Cytometry (FC/FACS) of anti-ADO antibody
ADO Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NCBI/Uniprot data below describe general gene information for ADO. It may not necessarily be applicable to this product.
NCBI Accession #
NP_116193.2
[Other Products]
NCBI GenBank Nucleotide #
NM_032804.5
[Other Products]
UniProt Primary Accession #
Q96SZ5
[Other Products]
UniProt Secondary Accession #
B1AL29[Other Products]
UniProt Related Accession #
Q96SZ5[Other Products]
NCBI Official Full Name
2-aminoethanethiol dioxygenase
NCBI Official Synonym Full Names
2-aminoethanethiol (cysteamine) dioxygenase
NCBI Official Symbol
ADO [Similar Products]
NCBI Official Synonym Symbols
C10orf22
[Similar Products]
NCBI Protein Information
2-aminoethanethiol dioxygenase
UniProt Protein Name
2-aminoethanethiol dioxygenase
UniProt Synonym Protein Names
Cysteamine dioxygenase
Protein Family
AdoMet-dependent rRNA methyltransferase
UniProt Gene Name
ADO [Similar Products]
UniProt Synonym Gene Names
C10orf22 [Similar Products]
UniProt Entry Name
AEDO_HUMAN
NCBI Summary for ADO
Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied by OMIM, Mar 2008]
UniProt Comments for ADO
ADO:
Protein type: Oxidoreductase; Other Amino Acids Metabolism - taurine and hypotaurine; EC 1.13.11.19
Chromosomal Location of Human Ortholog: 10q21.3
Cellular Component: mitochondrion
Molecular Function: metal ion binding; cysteamine dioxygenase activity
Product References and Citations for anti-ADO antibody
Dominy, J.E. Jr., et al. J. Biol. Chem. 282(35):25189-25198(2007)
Research Articles on ADO
1. Observational study of gene-disease association. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
