INSL3, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human INSL3. No significant cross-reactivity or interference between Human INSL3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human INSL3 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of INSL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2502152 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the INSL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INSL3. The ELISA analytical biochemical technique of the MBS2502152 kit is based on INSL3 antibody-INSL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INSL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INSL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of INSL3 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INSL3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human INSL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human INSL3. You can calculate the concentration of Human INSL3 in the samples by comparing the OD of the samples to the standard curve.

16,872 Da

insulin-like 3 (Leydig cell)

insulin-like 3; leydig insulin -like hormone; leydig insulin-like peptide; prepro-INSL3; relaxin-like factor b

Insulin-like 3

RLF; RLNL; Ley-I-L  [Similar Products]

INSL3_HUMAN

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2-p12

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: G-protein-coupled receptor binding; protease binding; hormone activity; insulin receptor binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; male gonad development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis

Disease: Cryptorchidism, Unilateral Or Bilateral

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