TPM2, Polyclonal Antibody

tropomyosin 2 (beta), DA1, DA2B, NEM4, TMSB, AMCD1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous level of total TPM2 protein.

Supplied at 1.5mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

Store at -20°C/1 year

Small volumes of anti-TPM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. TPM2 has been shown to interact with RRAD, PDLIM7 and TPM1.

Total protein Ab

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

ELISA: 1:1000-1:5000
Western blotting: 1:2000-1:5000
Immunohistochemistry: 1:50-1:200

Gel: 10%SDS-PAGE
Lysate: 40 ug Human cervical cancer tissue lysate
Primary antibody: 1/750 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP)
at 1/10000 dilution
Exposure time: 2 minutes

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MBS9406393 (TPM2 Antibody) at dilution 1/35, on the right is treated with the synthetic peptide.

tropomyosin 2 (beta)

tropomyosin beta chain

Tropomyosin beta chain

TMSB  [Similar Products]

TPM2_HUMAN

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

TPM2: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Defects in TPM2 are the cause of distal arthrogryposis type 1A (DA1A). A form of distal arthrogryposis, a disease characterized by congenital joint flexures or contractures that mainly involve the distal parts of the limbs, and affect two or more different body areas in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Belongs to the tropomyosin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of muscle; actin binding

Biological Process: regulation of ATPase activity; muscle contraction; muscle filament sliding

Disease: Nemaline Myopathy 4; Arthrogryposis, Distal, Type 1a; Myopathy, Congenital, With Fiber-type Disproportion; Arthrogryposis, Distal, Type 2b

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