Product Name
Beclin 2 (BECN2), Polyclonal Antibody
Full Product Name
Rabbit Anti-Human Beclin 2 Polyclonal
Product Synonym Names
BECN2 Antibody; Beclin-2 Antibody; Beclin-1 like protein 1 Antibody; Beclin 2 Antibody : ATTO 680; Rabbit Anti-Human Beclin 2 Polyclonal; Beclin 2 Antibody
Product Gene Name
anti-BECN2 antibody
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Matching Pairs
Unconjugated Antibody: N/A (MBS807726)
ATTO 680 Conjugated Antibody: Beclin 2 (MBS807726)
Matching Pairs
Unconjugated Antibody: Beclin 2 (MBS807761)
ATTO 680 Conjugated Antibody: Beclin 2 (MBS807726)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A8MW95
Species Reactivity
Human, Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the mid-protein of human Beclin 2
COA
A 1:1000 dilution was sufficient for detection of Beclin2 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Cellular Localization
Cytoplasm
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-BECN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BECN2 antibody
Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1).
Product Categories/Family for anti-BECN2 antibody
Cancer; Cell Signaling; Epigenetics; Autophagy
Applications Tested/Suitable for anti-BECN2 antibody
Western Blot (WB)
Application Notes for anti-BECN2 antibody
Western Blot: 1:1000
Western Blot (WB) of anti-BECN2 antibody
Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602). Lane 1: MW Ladder. Lane 2: Rat Liver (20 ug). Load: 20 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa.
NCBI/Uniprot data below describe general gene information for BECN2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277622.1
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NCBI GenBank Nucleotide #
NM_001290693.1
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UniProt Primary Accession #
A8MW95
[Other Products]
UniProt Secondary Accession #
E9KNW0[Other Products]
UniProt Related Accession #
A8MW95[Other Products]
Molecular Weight
Predicted molecular weight at ~48.1kDa. Observed bands in the 50-55kDa range, varied by lysates.
NCBI Official Full Name
beclin-2
NCBI Official Synonym Full Names
beclin 2
NCBI Official Symbol
BECN2 [Similar Products]
NCBI Official Synonym Symbols
BECN1L1; BECN1P1
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NCBI Protein Information
beclin-2
UniProt Protein Name
Beclin-2
UniProt Synonym Protein Names
Beclin-1 autophagy-related pseudogene 1
UniProt Gene Name
BECN2 [Similar Products]
UniProt Entry Name
BECN2_HUMAN
UniProt Comments for BECN2
BECN1L1: May play a role in autophagy and antiviral host defense (Potential). Belongs to the beclin family.
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: cytoplasmic membrane-bound vesicle
Molecular Function: protein binding; protein complex binding
Biological Process: autophagic vacuole formation; autophagy; cellular response to nitrogen starvation; CVT pathway; endosome to lysosome transport; glucose homeostasis; late endosome to vacuole transport
Product References and Citations for anti-BECN2 antibody
1. He C., et al. (2013) Cell. 154(5): 1085-1099.
Research Articles on BECN2
1. Study identified a previously uncharacterized mammalian-specific protein, Beclin 2, which, like Beclin 1, functions in autophagy and interacts with class III PI3K complex components.[Beclin 2]
Precautions
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Disclaimer
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