Product Name
KRT17, siRNA
Full Product Name
KRT17 siRNA (Rat)
Product Synonym Names
KA17; Keratin type I cytoskeletal 17; Cytokeratin-17; CK-17; Keratin-17; K17; Type I keratin Ka17
Product Gene Name
KRT17 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6IFU8
Specificity
KRT17 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KRT17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KRT17 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KRT17 sirna
siRNA to inhibit KRT17 expression using RNA interference
Applications Tested/Suitable for KRT17 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KRT17. It may not necessarily be applicable to this product.
NCBI Accession #
NP_997710.1
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NCBI GenBank Nucleotide #
NM_212545.2
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UniProt Primary Accession #
Q6IFU8
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UniProt Related Accession #
Q6IFU8[Other Products]
Molecular Weight
48,123 Da
NCBI Official Full Name
keratin, type I cytoskeletal 17
NCBI Official Synonym Full Names
keratin 17, type I
NCBI Official Symbol
Krt17 [Similar Products]
NCBI Official Synonym Symbols
Ka17
[Similar Products]
NCBI Protein Information
keratin, type I cytoskeletal 17
UniProt Protein Name
Keratin, type I cytoskeletal 17
UniProt Synonym Protein Names
Cytokeratin-17; CK-17; Keratin-17; K17; Type I keratin Ka17
UniProt Gene Name
Krt17 [Similar Products]
UniProt Synonym Gene Names
CK-17; K17 [Similar Products]
UniProt Entry Name
K1C17_RAT
UniProt Comments for KRT17
K17: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6.
Protein type: Cytoskeletal
Cellular Component: cytoplasm; keratin filament; intermediate filament
Molecular Function: MHC class II receptor activity; structural constituent of cytoskeleton; MHC class II protein binding
Biological Process: positive regulation of hair follicle development; hair follicle morphogenesis; positive regulation of translation; keratinization; morphogenesis of an epithelium; intermediate filament organization; intermediate filament-based process; signal transduction; positive regulation of cell growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
