Akt2, gamma, Polyclonal Antibody

Anti -Akt2, gamma (PKBb, RAC-PK Gamma 2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 7; NC_000073.5 (28376579..28424472). Location: 7 B1; 7 6.5 cM

Polyclonal

Rabbit

Recognizes both unphosphorylated and phosphorylated human AKT2. Species sequence homology: rat-92%, mouse-85%.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-Akt2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AKT2 is also known as V-AKT Murine Thymoma Viral Oncogene Homolog 2 antibody, Rac protein kinase beta antibody, PKB beta antibody or PRKBB antibody. AKT2 is an isoform of the phosphoinositide-dependent serine-threonine protein kinase AKT and is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of the hormone. AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Over-expression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several

Antibodies; Abs to Protein Kinases

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot (HEK293 cell lysate): 1:4000- 1:16,000 (60kD) 1ug AKT2 using TMB Stable Liquid Substrate for HRPO (3,3',5,5'-Tetramethylbenzidine) T6000 or T6005 as a substrate for 30 minutes at RT.

55,742 Da[Similar Products]

thymoma viral proto-oncogene 2

RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; OTTMUSP00000017783; OTTMUSP00000017784; OTTMUSP00000017785; OTTMUSP00000022681; protein kinase Akt-2; protein kinase B beta; protein kinase B, beta; RAC-beta serine/threonine protein kinase

MCG22777, isoform CRA_a

Q3TY95_MOUSE

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: EC 2.7.11.1; Oncoprotein; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; cell cortex; cytosol; nucleus; vesicle

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: glycogen metabolic process; positive regulation of positive chemotaxis; positive regulation of nitric oxide biosynthetic process; apoptosis; multicellular organismal development; positive regulation of glycogen biosynthetic process; intracellular protein transport across a membrane; positive regulation of vesicle fusion; negative regulation of caspase activity; protein amino acid phosphorylation; regulation of translation; positive regulation of glucose import; negative regulation of RNA splicing; transport; protein kinase B signaling cascade; glycogen biosynthetic process; positive regulation of signal transduction; carbohydrate transport; myelin maintenance in the peripheral nervous system; glucose metabolic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of fatty acid beta-oxidation; cellular response to insulin stimulus; carbohydrate metabolic process; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; phosphorylation; negative regulation of apoptosis; positive regulation of cell migration

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