Cdc6, Polyclonal Antibody

Cell division control protein 6 homolog; Cdc 18L; Cdc 6; CDC18 (cell division cycle 18, S.pombe, homolog) like; CDC18 (S.pombe); Cdc18 related protein; CDC18(S.pombe); Cdc18-related protein; Cdc18L; cdc6; CDC6 cell division cycle 6 homolog; CDC6 related protein; CDC6-related protein; CDC6_HUMAN; Cdc6p; Cell Cycle Controller; CELL CYCLE CONTROLLER CDC6; Cell division control protein 6; Cell division control protein 6 homolog; Cell Division Cycle 18; Cell division cycle 6 homolog (S. cerevisiae); Cell division cycle 6 homolog; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6); cell division cycle 6

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Immunogen Affinity Purified

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-CDC6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Cell division control protein 6 homolog(CDC6) detection. Tested with WB, IHC-P in Human;Rat.

Background: Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Anti- Cdc6 Picoband antibody, MBS178256, Western blotting
All lanes: Anti Cdc6 (MBS178256) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 63KD
Observed bind size: 63KD

Anti- Cdc6 Picoband antibody, MBS178256, IHC(P)
IHC(P): Rat Testis Tissue

Anti- Cdc6 Picoband antibody, MBS178256, IHC(P)
IHC(P): Human Mammary Cancer Tissue

62,720 Da

cell division cycle 6

cell division control protein 6 homolog

Cell division control protein 6 homolog

CDC18L; HsCdc18; HsCDC6  [Similar Products]

CDC6_HUMAN

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

CDC6: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1. Belongs to the CDC6/cdc18 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: cytoplasm; cytosol; Golgi apparatus; nucleoplasm; nucleus; spindle midzone; spindle pole

Molecular Function: ATP binding; kinase binding; nucleotide binding; protein binding

Biological Process: cell division; DNA replication; DNA replication checkpoint; DNA replication initiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; mitosis; negative regulation of cell proliferation; negative regulation of DNA replication; positive regulation of chromosome segregation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of cytokinesis; positive regulation of fibroblast proliferation; regulation of cyclin-dependent protein kinase activity; regulation of mitotic metaphase/anaphase transition; traversing start control point of mitotic cell cycle

Disease: Meier-gorlin Syndrome 5

1. Gonzalez, S., Klatt, P., Delgado, S., Conde, E., Lopez-Rios, F., Sanchez-Cespedes, M., Mendez, J., Antequera, F., Serrano, M. Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Mimura, S., Seki, T., Tanaka, S., Diffley, J. F. X. Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control. Nature 431: 1118-1123, 2004. 3. Whitmire, E., Khan, B., Coue, M. Cdc6 synthesis regulates replication competence in Xenopus oocytes. Nature 419: 722-725, 2002.

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