Wingless-type MMTV integration site family, member 4, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of WNT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS744761 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless-type MMTV integration site family, member 4 (WNT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT4. The ELISA analytical biochemical technique of the MBS744761 kit is based on WNT4 antibody-WNT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: WNT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-WNT4 antibody and an WNT4-HRP conjugate. The assay sample and buffer are incubated together with WNT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WNT4 concentration since WNT4 from samples and WNT4-HRP conjugate compete for the anti-WNT4 antibody . Since the number of sites is limited, as more sites are occupied by WNT4 from the sample, fewer sites are left to bind WNT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WNT4 concentration in each sample is interpolated from this standard curve.

Signal Transduction

32,954 Da

wingless-type MMTV integration site family, member 4

protein Wnt-4

Protein Wnt-4

UNQ426/PRO864  [Similar Products]

WNT4_HUMAN

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.23-p35.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region

Molecular Function: frizzled binding; transcription corepressor activity; receptor agonist activity

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; thyroid stimulating hormone secreting cell differentiation; Wnt receptor signaling pathway through beta-catenin; androgen biosynthetic process; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; positive regulation of focal adhesion formation; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; kidney development; negative regulation of cell migration; female gonad development; cell fate commitment; positive regulation of meiosis; adrenal gland development; male gonad development; immature T cell proliferation in the thymus; positive regulation of bone mineralization; liver development; cellular response to starvation; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; ureteric bud branching; epithelial to mesenchymal transition; smooth muscle cell differentiation; female sex determination; negative regulation of transcription, DNA-dependent; oocyte development

Disease: Mullerian Aplasia And Hyperandrogenism; 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs

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