Butyrylcholinesterase (BCHE), ELISA Kit

BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Butyrylcholinesterase (BCHE).
No significant cross-reactivity or interference between Butyrylcholinesterase (BCHE) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BCHE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2700570 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Butyrylcholinesterase (BCHE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BCHE. The ELISA analytical biochemical technique of the MBS2700570 kit is based on BCHE antibody-BCHE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BCHE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BCHE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Butyrylcholinesterase (BCHE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Butyrylcholinesterase (BCHE) and unlabeled Butyrylcholinesterase (BCHE) (Standards or samples) with the pre-coated antibody specific to Butyrylcholinesterase (BCHE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Butyrylcholinesterase (BCHE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Butyrylcholinesterase (BCHE) in the sample.

68,418 Da

butyrylcholinesterase

cholinesterase

Cholinesterase

CHE1  [Similar Products]

This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: EC 3.1.1.8; Hydrolase; Nuclear envelope; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: endoplasmic reticulum lumen; extracellular region; membrane; nuclear envelope lumen

Molecular Function: acetylcholinesterase activity; amyloid-beta binding; catalytic activity; choline binding; cholinesterase activity; enzyme binding; hydrolase activity, acting on ester bonds; identical protein binding

Biological Process: choline metabolic process; cocaine metabolic process; learning; negative regulation of cell proliferation; negative regulation of synaptic transmission; neuroblast differentiation; response to alkaloid; response to drug; response to folic acid; response to glucocorticoid stimulus

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.