Product Name
ATP8B1, siRNA
Full Product Name
ATP8B1 siRNA (Mouse)
Product Synonym Names
Probable phospholipid-transporting ATPase IC; ATPase class I type 8B member 1
Product Gene Name
ATP8B1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q148W0
Specificity
ATP8B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP8B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP8B1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP8B1 sirna
siRNA to inhibit ATP8B1 expression using RNA interference
Applications Tested/Suitable for ATP8B1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP8B1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001001488.2
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NCBI GenBank Nucleotide #
NM_001001488.3
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UniProt Primary Accession #
Q148W0
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UniProt Secondary Accession #
Q3U010; Q6R964[Other Products]
UniProt Related Accession #
Q148W0[Other Products]
Molecular Weight
143,798 Da
NCBI Official Full Name
phospholipid-transporting ATPase IC
NCBI Official Synonym Full Names
ATPase, class I, type 8B, member 1
NCBI Official Symbol
Atp8b1 [Similar Products]
NCBI Official Synonym Symbols
Ic; FIC1; AI451886
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NCBI Protein Information
phospholipid-transporting ATPase IC
UniProt Protein Name
Phospholipid-transporting ATPase IC
UniProt Synonym Protein Names
ATPase class I type 8B member 1; P4-ATPase flippase complex alpha subunit ATP8B1
Protein Family
Phospholipid-transporting ATPase
UniProt Gene Name
Atp8b1 [Similar Products]
UniProt Entry Name
AT8B1_MOUSE
UniProt Comments for ATP8B1
ATP8B1: May play a role in the transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids in the canicular membrane. May have a role in transport of bile acids into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both. Defects in ATP8B1 are the cause of progressive familial intrahepatic cholestasis type 1 (PFIC1); also known as Byler disease. PFIC1 is an autosomal recessive disorder, characterized by early infancy cholestasis, that may be initially episodic but progresses to malnutrition, growth retardation and end-stage liver disease before *****hood. Defects in ATP8B1 are the cause of benign recurrent intrahepatic cholestasis type 1 (BRIC1); also known as Summerskill syndrome. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration. Patients are asymptomatic between episodes, both clinically and biochemically. Defects in ATP8B1 can be associated with intrahepatic cholestasis of pregnancy (ICP); also known as pregnancy-related cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.
Protein type: Hydrolase; Membrane protein, multi-pass; Transporter; EC 3.6.3.1; Transporter, ion channel; Membrane protein, integral
Cellular Component: Golgi apparatus; stereocilium; cell projection; membrane; endoplasmic reticulum; brush border membrane; apical plasma membrane; plasma membrane; integral to membrane
Molecular Function: phospholipid-translocating ATPase activity; hydrolase activity; metal ion binding; magnesium ion binding; nucleotide binding; ATP binding
Biological Process: phospholipid translocation; sensory perception of sound; vestibulocochlear nerve formation; bile acid metabolic process; inner ear receptor cell development; transport; phospholipid transport; multidrug transport; negative regulation of transcription, DNA-dependent; lipid transport; Golgi organization and biogenesis; regulation of microvillus biogenesis
Research Articles on ATP8B1
1. The authors show that two of these transporters, ABCB11 and ATP8B1, are functional targets of miR-33, a micro-RNA that is expressed from within an intron of SREBP-2.
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