CDC6, Polyclonal Antibody

Cdc 18L; Cdc 6; CDC18 (cell division cycle 18, S.pombe, homolog) like; CDC18 (S.pombe); Cdc18 related protein; CDC18(S.pombe); Cdc18-related protein; Cdc18L; cdc6; CDC6 cell division cycle 6 homolog; CDC6 related protein; CDC6-related protein; CDC6_HUMAN; Cdc6p; CELL CYCLE CONTROLLER CDC6; Cell division control protein 6; Cell division control protein 6 homolog; Cell division cycle 6 homolog (S. cerevisiae); Cell division cycle 6 homolog; Cell division cycle 6, S. cerevisiae, homolog of; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

CDC6 antibody detects endogenous levels of total CDC6

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-CDC6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Function: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subunit Structure: Interacts with PCNA, ORC1, cyclin-CDK (PubMed:9566895). Interacts with HUWE1 (PubMed:17567951). Interacts with ANKRD17 (PubMed:23711367). Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6 (PubMed:25990725). Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin (PubMed:14672932).
Similarity: Belongs to the CDC6/cdc18 family.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

WB: 1:1000-3000
IF/ICC: 1:100-1:500

Western blot analysis of CDC6 using RAW264.7 whole lysates

MBS9608382 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

Observed: 63 kDa
Predicted: 63 kDa

cell division cycle 6

cell division control protein 6 homolog

Cell division control protein 6 homolog

CDC18L; HsCdc18; HsCDC6  [Similar Products]

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

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