ALDOB, Recombinant Protein

Fructose-bisphosphate aldolase B; ALDB; ALDO2

For Research Use Only. Not for use in diagnostic procedures.

E Coli
Source: Human

> 95%

Liquid
In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT; 10% glycerol; 0.1 M NaCl

Store at -20 degree C for 12 months.
Centrifuge the vial prior to opening.

Small volumes of ALDOB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Fructose-bisphosphate aldolase B; also known as ALDOB; is one of three known aldolase isoenzymes; and is found in kidney and small ***** intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein; fused to His-tag at N-terminus; was expressed in E Coli and purified by using conventional chromatography techniques.

SDS-PAGE

42 kDa

aldolase, fructose-bisphosphate B

fructose-bisphosphate aldolase B

Fructose-bisphosphate aldolase B

ALDB  [Similar Products]

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in ***** muscle where it can be as much as 5% of total cellular protein. In ***** liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: cytosol; microtubule organizing center

Molecular Function: ATPase binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; identical protein binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose metabolic process; gluconeogenesis; glycolysis; NADH oxidation; positive regulation of ATPase activity

Disease: Fructose Intolerance, Hereditary

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