Product Name
NaVbeta3, Monoclonal Antibody
Full Product Name
NaVbeta3 Antibody, Clone S396-29: Dylight 633
Product Synonym Names
Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B; Nav beta3 Antibody: Dylight 633; Sodium channel subunit beta-3 Antibody; Scn3b Antibody; KIAA1158 Antibody
Product Gene Name
anti-NaVbeta3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JK00
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~40 kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.1% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
Cellular Localization
Membrane
Certificate of Analysis
A 1:100 dilution was sufficient for detection of NavBeta3 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-NaVbeta3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NaVbeta3 antibody
Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).
Product Categories/Family for anti-NaVbeta3 antibody
Neuroscience; Ion Channels; Sodium Channels; Voltage-Gated Sodium Channels
Applications Tested/Suitable for anti-NaVbeta3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NaVbeta3 antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-NaVbeta3 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta 3 Monoclonal Antibody, Clone S396-29 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Nav beta 3 Antibody. (D) Composite.
Western Blot (WB) of anti-NaVbeta3 antibody
Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29 at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.
NCBI/Uniprot data below describe general gene information for NaVbeta3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_620797.1
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NCBI GenBank Nucleotide #
NP_620797.1
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UniProt Primary Accession #
Q9JK00
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UniProt Related Accession #
Q9JK00[Other Products]
NCBI Official Full Name
sodium channel subunit beta-3
NCBI Official Synonym Full Names
sodium voltage-gated channel beta subunit 3
NCBI Official Symbol
Scn3b [Similar Products]
NCBI Official Synonym Symbols
Scnb3
[Similar Products]
NCBI Protein Information
sodium channel subunit beta-3
UniProt Protein Name
Sodium channel subunit beta-3
UniProt Gene Name
Scn3b [Similar Products]
UniProt Entry Name
SCN3B_RAT
NCBI Summary for NaVbeta3
voltage-sensitive sodium channel auxiliary subunit; modulates channel gating with distinct kinetics [RGD, Feb 2006]
UniProt Comments for NaVbeta3
SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.
Protein type: Channel, sodium; Membrane protein, integral
Cellular Component: integral to membrane; plasma membrane; voltage-gated sodium channel complex; Z disc
Molecular Function: sodium channel regulator activity
Biological Process: cardiac muscle contraction; membrane depolarization; nervous system development; positive regulation of heart rate; sensory perception of pain; sodium ion transport
Product References and Citations for anti-NaVbeta3 antibody
1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015. 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.
Research Articles on NaVbeta3
1. the presences of Nav1.1, Nav1.6, Navbeta1 and Navbeta3 mRNA and their reduced levels in rat SAN during aging.
Precautions
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Disclaimer
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