Product Name
SCN3B, siRNA
Full Product Name
SCN3B siRNA (Human)
Product Synonym Names
KIAA1158; Sodium channel subunit beta-3
Product Gene Name
SCN3B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NY72
Specificity
SCN3B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SCN3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SCN3B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SCN3B sirna
siRNA to inhibit SCN3B expression using RNA interference
Applications Tested/Suitable for SCN3B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SCN3B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001035241.1
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NCBI GenBank Nucleotide #
NM_001040151.1
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UniProt Primary Accession #
Q9NY72
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UniProt Secondary Accession #
Q17RL3; Q9ULR2; A5H1I5[Other Products]
UniProt Related Accession #
Q9NY72[Other Products]
Molecular Weight
24,702 Da
NCBI Official Full Name
sodium channel subunit beta-3
NCBI Official Synonym Full Names
sodium channel, voltage gated, type III beta subunit
NCBI Official Symbol
SCN3B [Similar Products]
NCBI Official Synonym Symbols
SCNB3; ATFB16; BRGDA7; HSA243396
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NCBI Protein Information
sodium channel subunit beta-3
UniProt Protein Name
Sodium channel subunit beta-3
Protein Family
Sodium channel
UniProt Gene Name
SCN3B [Similar Products]
UniProt Synonym Gene Names
KIAA1158 [Similar Products]
UniProt Entry Name
SCN3B_HUMAN
NCBI Summary for SCN3B
Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for SCN3B
SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.
Protein type: Channel, sodium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: voltage-gated sodium channel complex; plasma membrane; integral to membrane; Z disc
Molecular Function: sodium channel regulator activity; voltage-gated sodium channel activity
Biological Process: nervous system development; membrane depolarization; sodium ion transport; positive regulation of heart rate; sensory perception of pain; cardiac muscle contraction
Disease: Brugada Syndrome 7
Research Articles on SCN3B
1. SCN1-3B the gene that encode respectively a- and b-subunits of the cardiac fast voltage-gated sodium channel, have been linked to atrial fibrillation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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