Product Name
Keratin 17 (KRT17), Polyclonal Antibody
Full Product Name
Keratin 17 Rabbit pab Antibody
Product Synonym Names
KRT17; Keratin 17 ; Keratin 17 ; RT17; K17; PC; PC2; PCHC1
Product Gene Name
anti-KRT17 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (39775692..39780882, complement). Location: 17q21.2
3D Structure
ModBase 3D Structure for Q04695
Species Reactivity
Human, Rat
Purity/Purification
Affinity purification
Immunogen
A synthetic -peptide of human Keratin 17
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Other Notes
Small volumes of anti-KRT17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT17 antibody
Rabbit polyclonal to Keratin 17
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling (7). Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3sigma localization. As 14-3-3sigma typically associates with keratin 17, these results imply that Akt/mTOR signaling results in sequestration of 14-3-3sigma with keratin 17 in the cytosol, which is required for translation and cell growth. Phosphorylation of keratin 17 on Ser44 may provide a docking site for 14-3-3sigma binding (8).
Applications Tested/Suitable for anti-KRT17 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-KRT17 antibody
Western Blot (WB): 1/500-1000
Immunohistochemistry (IHC): 1/50
Immunofluorescence (IF): 1/250
Western Blot (WB) of anti-KRT17 antibody
Western blot analysis of extracts of various cell lines, using KRT17 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry (IHC) of anti-KRT17 antibody
Immunohistochemistry of paraffin-embedded rat kidney using KRT17 antibody at dilution of 1:200 (40x lens).
NCBI/Uniprot data below describe general gene information for KRT17. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000413.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000422.2
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UniProt Primary Accession #
Q04695
[Other Products]
UniProt Secondary Accession #
Q6IP98; Q8N1P6; A5Z1M9; A5Z1N0; A5Z1N1; A5Z1N2; A6NDV6; A6NKQ2[Other Products]
UniProt Related Accession #
Q04695[Other Products]
Molecular Weight
432[Similar Products]
NCBI Official Full Name
keratin, type I cytoskeletal 17
NCBI Official Synonym Full Names
keratin 17
NCBI Official Symbol
KRT17 [Similar Products]
NCBI Official Synonym Symbols
PC; K17; PC2; PCHC1
[Similar Products]
NCBI Protein Information
keratin, type I cytoskeletal 17; 39.1; CK-17; keratin-17; cytokeratin-17; keratin 17 epitope S1; keratin 17 epitope S2; keratin 17 epitope S4
UniProt Protein Name
Keratin, type I cytoskeletal 17
UniProt Synonym Protein Names
39.1; Cytokeratin-17; CK-17; Keratin-17
UniProt Gene Name
KRT17 [Similar Products]
UniProt Synonym Gene Names
CK-17; K17 [Similar Products]
UniProt Entry Name
K1C17_HUMAN
Product References and Citations for anti-KRT17 antibody
1. Moll, R. et al. (1982) Cell 31, 11-24. 2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. 4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. 5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76. 6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. 7. Paladini, R.D. et al. (1996) J. Cell Biol. 132, 381-397.
Precautions
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Disclaimer
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