Glypican 5, Monoclonal Antibody

Anti -Glypican 5 (Glypiated Proteoglycan 5) (Carboxyfluorescein)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 13; NC_000013.10 (92050935..93519490). Location: 13q32

Monoclonal

IgG2a

7H18

Mouse

Recognizes human/mouse Glypican 5.

Purified
Purified immunoglobulin

Supplied as a liquid in PBS, BSA. Labeled with Carboxyfluorescein.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-GPC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are currently six known mammalian glypicans. They all share a common-sized protein core of 60-70 kD, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing Glypicans 1, 2, 4, and 6, and another subfamily containing Glypican 3 and 5 (3, 5). Human Glypican 5 (GPC 5) is synthesized as a 572 amino acid (aa) preproprecursor that contains a 24 aa signal sequence, a 532 aa mature region and a 16 aa C-terminal pro-segment (6, 7). There are three potential N-linked, and five potential O-linked sites for glycosylation or glycanation. GPC 5 is believed to contain 6-7 kD of glycosylation and at least 55 kD of proteoglycan. This is based on an assumption of the presence of one heparan sulfate chain of 36 kD and one chrondroitin sulfate chain of 17 kD (7, 8). When added to the core molecular weight of 59 kD, the mature protein is approximately 120 kD in size. To date, however, the actual size of native human GPC 5 has not been reported and the suggestion of a chrondroitin sulfate modification is based on the expression of human GPC 5 in COS-7 cells (7). Human to mouse, there is 88% aa identity over the mature region. Cells known to express GPC 5 are principally embryonic in nature, and include neurons and mesenchyme (1, 7). The function of GPC 5 is essentially unknown. As a glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (9, 10).

Antibodies; Abs to Binding Proteins

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry.

63,707 Da[Similar Products]

glypican 5

glypican-5; bA93M14.1; OTTHUMP00000018551; glypican proteoglycan 5

Glypican-5

GPC5_HUMAN

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

GPC5: Cell surface proteoglycan that bears heparan sulfate. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Cell surface

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; extracellular space; Golgi lumen; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

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