GLuD1, Recombinant Protein

Glutamate dehydrogenase 1 mitochondrial; Glutamate dehydrogenase 1; mitochondrial; GDH; GDH1; GLuD

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 80% by SDS-PAGE

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol

1mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of GLuD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Metabolism

SDS-PAGE

58.4 kDa (528aa)

glutamate dehydrogenase 1

glutamate dehydrogenase 1, mitochondrial

Glutamate dehydrogenase 1, mitochondrial

GLUD; GDH 1  [Similar Products]

This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate ().

Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516.; Tanizawa Y., et al. (2002) Diabetes. 51:712-717.;

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.