Glutamate Dehydrogenase 1, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS741776 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamate Dehydrogenase 1 (GDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GDH. The ELISA analytical biochemical technique of the MBS741776 kit is based on GDH antibody-GDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

61,398 Da

glutamate dehydrogenase 1

glutamate dehydrogenase 1, mitochondrial; GDH 1; glutamate dehydrogenase (NAD(P)+)

Glutamate dehydrogenase 1, mitochondrial

GLUD; GDH 1  [Similar Products]

DHE3_HUMAN

This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009]

GLUD1: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Homohexamer. Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH and activate the enzyme. Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Protein type: Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline; EC 1.4.1.3; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm

Molecular Function: identical protein binding; protein binding; glutamate dehydrogenase activity; GTP binding; glutamate dehydrogenase [NAD(P)+] activity; ADP binding; ATP binding

Biological Process: glutamate catabolic process; substantia nigra development; positive regulation of insulin secretion; glutamine metabolic process; amino acid biosynthetic process; glutamate biosynthetic process

Disease: Hyperinsulinemic Hypoglycemia, Familial, 6

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