Full Product Name
TTC8 siRNA (Human)
Product Synonym Names
BBS8; Tetratricopeptide repeat protein 8; TPR repeat protein 8; Bardet-Biedl syndrome 8 protein
Product Gene Name
TTC8 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8TAM2
Specificity
TTC8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TTC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TTC8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TTC8 sirna
siRNA to inhibit TTC8 expression using RNA interference
Applications Tested/Suitable for TTC8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TTC8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001275710.1
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NCBI GenBank Nucleotide #
NM_001288781.1
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UniProt Primary Accession #
Q8TAM2
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UniProt Secondary Accession #
Q67B97; Q86SY0; Q86TV9; Q86U26; Q8NDH9; Q96DG8; A6NFG2; B3KWA5[Other Products]
UniProt Related Accession #
Q8TAM2[Other Products]
Molecular Weight
21,712 Da
NCBI Official Full Name
tetratricopeptide repeat protein 8 isoform D
NCBI Official Synonym Full Names
tetratricopeptide repeat domain 8
NCBI Official Symbol
TTC8 [Similar Products]
NCBI Official Synonym Symbols
BBS8; RP51
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NCBI Protein Information
tetratricopeptide repeat protein 8
UniProt Protein Name
Tetratricopeptide repeat protein 8
UniProt Synonym Protein Names
Bardet-Biedl syndrome 8 protein
UniProt Gene Name
TTC8 [Similar Products]
UniProt Synonym Gene Names
BBS8; TPR repeat protein 8 [Similar Products]
UniProt Entry Name
TTC8_HUMAN
NCBI Summary for TTC8
This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for TTC8
TTC8: The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Defects in TTC8 are the cause of retinitis pigmentosa type 51 (RP51). It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in TTC8 are the cause of Bardet-Biedl syndrome type 8 (BBS8). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 14q31.3
Cellular Component: centrosome; cytosol; photoreceptor connecting cilium; cilium
Molecular Function: protein binding
Biological Process: fat cell differentiation; axon guidance; protein transport; sensory processing; regulation of protein localization; sensory cilium biogenesis; organelle organization and biogenesis; establishment of anatomical structure orientation; multicellular organism growth; olfactory bulb development; sensory perception of smell; cilium biogenesis
Disease: Retinitis Pigmentosa 51; Bardet-biedl Syndrome 8
Research Articles on TTC8
1. Observational study of genetic testing. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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