Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD), ELISA Kit

CD240D; Rh30a; Rh4; RhPI; RhII; DIIIc; Rhesus D antigen; Rh polypeptide 2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD).
No significant cross-reactivity or interference between Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RHD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2097747 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RHD. The ELISA analytical biochemical technique of the MBS2097747 kit is based on RHD antibody-RHD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RHD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RHD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Hematology

41,694 Da

Rh blood group D antigen

blood group Rh(D) polypeptide

Blood group Rh(D) polypeptide

RhPII  [Similar Products]

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

RHD: May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: integral component of plasma membrane

Molecular Function: ammonium transmembrane transporter activity

Biological Process: organic cation transport

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