Product Name
RPS14, siRNA
Full Product Name
RPS14 siRNA (Human)
Product Synonym Names
40S ribosomal protein S14
Product Gene Name
RPS14 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62263
Specificity
RPS14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPS14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RPS14 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RPS14 sirna
siRNA to inhibit RPS14 expression using RNA interference
Applications Tested/Suitable for RPS14 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RPS14. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001020241.1
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NCBI GenBank Nucleotide #
NM_001025070.1
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UniProt Primary Accession #
P62263
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UniProt Secondary Accession #
P06366; Q5BJI0; B2R5G5; D3DQG5[Other Products]
UniProt Related Accession #
P62263[Other Products]
Molecular Weight
16,273 Da
NCBI Official Full Name
40S ribosomal protein S14
NCBI Official Synonym Full Names
ribosomal protein S14
NCBI Official Symbol
RPS14 [Similar Products]
NCBI Official Synonym Symbols
S14; EMTB
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NCBI Protein Information
40S ribosomal protein S14
UniProt Protein Name
40S ribosomal protein S14
Protein Family
Ribosomal protein
UniProt Gene Name
RPS14 [Similar Products]
UniProt Entry Name
RS14_HUMAN
NCBI Summary for RPS14
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for RPS14
RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Protein type: Translation; RNA-binding; Ribosomal
Chromosomal Location of Human Ortholog: 5q31-q33
Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; cytosol
Molecular Function: structural constituent of ribosome; RNA binding; translation regulator activity; mRNA 5'-UTR binding
Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; negative regulation of transcription from RNA polymerase II promoter; viral infectious cycle; translational termination; maturation of SSU-rRNA; regulation of translation; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; erythrocyte differentiation; viral transcription; gene expression
Disease: Chromosome 5q Deletion Syndrome
Research Articles on RPS14
1. Partial silencing of RPS14 inhibits the proliferation of SKM-1, an acute myeloid leukemia cell line, and RPS14 negatively regulates p53 activation in SKM-1 cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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