MAGEA2, Polyclonal Antibody

CT1.2; MAGE2; MAGEA2A

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total MAGEA2 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

1.4 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-MAGEA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene.

Total protein Ab

Western Blot (WB)

Western blotting: 1:200-1:1000

Gel: 10%SDS-PAGE Lysates (from left to right): Human leiomyosarcoma tissue Amount of lysate: 40ug per lane Primary antibody: 1/700 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds

35,055 Da

melanoma antigen family A2

melanoma-associated antigen 2

Melanoma-associated antigen 2

MAGE2; MAGEA2A; CT1.2  [Similar Products]

MAGA2_HUMAN

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

MAGE-A2: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T- lymphocytes.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: PML body; nucleus

Molecular Function: protein binding; histone deacetylase binding; ubiquitin protein ligase binding

Biological Process: transcription, DNA-dependent; cellular protein catabolic process; positive regulation of ubiquitin-protein ligase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein sumoylation

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