pseudouridylate synthase 1, ELISA Kit

Human tRNA pseudouridine synthase A; mitochondrial (PUS1) ELISA kit; MGC11268; pseudouridine synthase 1; tRNA uridine isomerase I; pseudouridylate synthase 1

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PUS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9334504 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pseudouridylate synthase 1 (PUS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PUS1. The ELISA analytical biochemical technique of the MBS9334504 kit is based on PUS1 antibody-PUS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PUS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PUS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

47,470 Da

pseudouridylate synthase 1

tRNA pseudouridine synthase A, mitochondrial; tRNA uridine isomerase I; tRNA-uridine isomerase I; tRNA pseudouridylate synthase I; tRNA pseudouridine(38-40) synthase; mitochondrial tRNA pseudouridine synthase A

tRNA pseudouridine synthase A, mitochondrial

PP8985  [Similar Products]

TRUA_HUMAN

This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

PUS1: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA. Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1); also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Belongs to the tRNA pseudouridine synthase TruA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.4.99.12; RNA-binding; Isomerase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; RNA processing

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: transcription factor complex; mitochondrion; nuclear chromatin; nucleolus; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; pseudouridine synthase activity; pseudouridylate synthase activity

Biological Process: tRNA pseudouridine synthesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Myopathy, Lactic Acidosis, And Sideroblastic Anemia 1

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.