Full Product Name
APLF Polyclonal Antibody
Product Synonym Names
APFL; PALF; Xip1; C2orf13
Product Gene Name
anti-APLF antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen affinity purification
Concentration
1.8mg/mL (lot specific)
Immunogen
Recombinant protein of human APLF
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-APLF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APLF antibody
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks. Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro.
Applications Tested/Suitable for anti-APLF antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-APLF antibody
IHC: 1:50-1:200
Immunohistochemistry (IHC) of anti-APLF antibody
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using APLF Polyclonal Antibody at dilution 1:40
Immunohistochemistry (IHC) of anti-APLF antibody
Immunohistochemistry of paraffin-embedded Human brain tissue using APLF Polyclonal Antibody at dilution 1:40
NCBI/Uniprot data below describe general gene information for APLF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_775816.1
[Other Products]
NCBI GenBank Nucleotide #
NM_173545.2
[Other Products]
UniProt Secondary Accession #
Q53P47; Q53PB9; Q53QU0; A8K476[Other Products]
UniProt Related Accession #
Q8IW19[Other Products]
Molecular Weight
56,956 Da
NCBI Official Full Name
aprataxin and PNK-like factor
NCBI Official Synonym Full Names
aprataxin and PNKP like factor
NCBI Official Symbol
APLF [Similar Products]
NCBI Official Synonym Symbols
APFL; PALF; Xip1; ZCCHH1; C2orf13
[Similar Products]
NCBI Protein Information
aprataxin and PNK-like factor; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; aprataxin- and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; zinc finger, CX5CX6HX5H motif containing 1
UniProt Protein Name
Aprataxin and PNK-like factor
UniProt Synonym Protein Names
Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1
Protein Family
Aprataxin and PNK-like factor
UniProt Gene Name
APLF [Similar Products]
UniProt Synonym Gene Names
C2orf13; PALF; XIP1 [Similar Products]
UniProt Entry Name
APLF_HUMAN
NCBI Summary for APLF
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008]
UniProt Comments for APLF
APLF: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5. Belongs to the APLF family.
Protein type: DNA repair, damage; EC 4.2.99.18
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: nucleoplasm; cytosol; nucleus
Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; metal ion binding; nucleotide binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity
Biological Process: regulation of isotype switching; single strand break repair; double-strand break repair; positive regulation of DNA ligation; DNA catabolic process, endonucleolytic; response to DNA damage stimulus
Research Articles on APLF
1. These data suggest functional requirements for Ku and XRCC4 in APLF-dependent NHEJ and a unique role for Ku as a factor required to facilitate the nuclear retention of APLF.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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