Nav beta 3, Monoclonal Antibody

Sodium channel subunit beta-3 Antibody; Scn3b Antibody; KIAA1158 Antibody; Nav beta 3 Antibody: Streptavidin; Mouse Anti-Rat NaVbeta3 Monoclonal, Clone S396-29; Anti-Nav beta 3; Nav beta 3 Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2B

S396-29

Mouse

Protein G Purified

1 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-Nav beta 3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).

Neuroscience; Ion Channels; Sodium Channels; Voltage-Gated Sodium Channels

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot: 1:1000

Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.

Detects ~40kDa.

sodium voltage-gated channel beta subunit 3

sodium channel subunit beta-3

Sodium channel subunit beta-3

SCN3B_RAT

voltage-sensitive sodium channel auxiliary subunit; modulates channel gating with distinct kinetics [RGD, Feb 2006]

SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.

Protein type: Channel, sodium; Membrane protein, integral

Cellular Component: integral to membrane; plasma membrane; voltage-gated sodium channel complex; Z disc

Molecular Function: sodium channel regulator activity

Biological Process: cardiac muscle contraction; membrane depolarization; nervous system development; positive regulation of heart rate; sensory perception of pain; sodium ion transport

1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015, from http://www.ncbi.nlm.nih.gov/gene/?term=Q9jK00 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.

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