Adenylyl cyclase-associated protein 1, ELISA Kit

RP11-115D7.1; CAP; CAP1-PEN; OTTHUMP00000004822|adenylyl cyclase-associated protein

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat CAP1. No significant cross-reactivity or interference between Rat CAP1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284368 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenylyl cyclase-associated protein 1 (CAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CAP1. The ELISA analytical biochemical technique of the MBS284368 kit is based on CAP1 antibody-CAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CAP1 in samples. An antibody specific for CAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

51,589 Da

cyclase associated actin cytoskeleton regulatory protein 1

adenylyl cyclase-associated protein 1

Adenylyl cyclase-associated protein 1

Cap; Mch1; CAP 1  [Similar Products]

associated with the S. cerevisia adenylyl cyclase-assoiciated protein (CAP); may be involved in the cyclic AMP signalling pathway [RGD, Feb 2006]

CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q36

Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; plasma membrane

Molecular Function: actin binding; adenylate cyclase binding

Biological Process: actin cytoskeleton organization; actin polymerization or depolymerization; ameboidal cell migration; cell morphogenesis; establishment and/or maintenance of cell polarity; receptor-mediated endocytosis; regulation of adenylate cyclase activity; signal transduction

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