Full Product Name
TBX1 siRNA (Human)
Product Synonym Names
T-box transcription factor TBX1; T-box protein 1; Testis-specific T-box protein
Product Gene Name
TBX1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43435
Specificity
TBX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TBX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TBX1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TBX1 sirna
siRNA to inhibit TBX1 expression using RNA interference
Applications Tested/Suitable for TBX1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TBX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005983.1
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NCBI GenBank Nucleotide #
NM_005992.1
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UniProt Primary Accession #
O43435
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UniProt Secondary Accession #
O43436; Q96RJ2; C6G493; C6G494[Other Products]
UniProt Related Accession #
O43435[Other Products]
Molecular Weight
52,666 Da
NCBI Official Full Name
T-box transcription factor TBX1 isoform B
NCBI Official Synonym Full Names
T-box 1
NCBI Official Symbol
TBX1 [Similar Products]
NCBI Official Synonym Symbols
DGS; TGA; VCF; CAFS; CTHM; DGCR; DORV; VCFS; TBX1C; CATCH22
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NCBI Protein Information
T-box transcription factor TBX1
UniProt Protein Name
T-box transcription factor TBX1
UniProt Synonym Protein Names
Testis-specific T-box protein
Protein Family
T-box transcription factor
UniProt Gene Name
TBX1 [Similar Products]
UniProt Synonym Gene Names
T-box protein 1 [Similar Products]
UniProt Entry Name
TBX1_HUMAN
NCBI Summary for TBX1
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TBX1
TBX1: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS). DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life. Defects in TBX1 are a cause of DiGeorge syndrome (DGS). Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS). Defects in TBX1 are a cause of conotruncal heart malformations (CTHM). CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: nucleus
Molecular Function: protein dimerization activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: retinoic acid receptor signaling pathway; heart morphogenesis; muscle development; heart development; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; cell fate specification; ear morphogenesis; middle ear morphogenesis; anterior/posterior pattern formation; sensory perception of sound; positive regulation of MAPKKK cascade; epithelial cell differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; thyroid gland development; mesoderm development; angiogenesis; neural crest cell migration; muscle morphogenesis; blood vessel development; inner ear morphogenesis; tongue morphogenesis; pharyngeal system development; thymus development; transcription, DNA-dependent; outer ear morphogenesis; semicircular canal morphogenesis; embryonic cranial skeleton morphogenesis; social behavior; embryonic viscerocranium morphogenesis; pattern specification process; parathyroid gland development; odontogenesis of dentine-containing teeth; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of cell differentiation; artery morphogenesis; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; soft palate development; positive regulation of protein amino acid phosphorylation; lymph vessel development; determination of left/right symmetry; vagus nerve morphogenesis; positive regulation of epithelial cell proliferation
Disease: Conotruncal Heart Malformations; Digeorge Syndrome; Velocardiofacial Syndrome; Tetralogy Of Fallot
Research Articles on TBX1
1. SNPs in three genes CYP26B1 rs2241057, CISD1 rs2251039, rs2590370, and TBX1 rs4819522 were involved in six potential pathways to influence serum prostate-specific antigen levels.
Precautions
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