Product Name
Cav beta 4 (CACNB4), Monoclonal Antibody
Full Product Name
Cav beta 4 Antibody, Clone S10-7: Dylight 488
Product Synonym Names
Mouse Anti-Rat Cav beta 4 Monoclonal IgG1; Cav beta 4 Antibody: Dylight 488; CAB4 Antibody; CACNLB4 Antibody; EA5 Antibody; EJM Antibody; Calcium channel voltage dependent beta 4 subunit Antibody
Product Gene Name
anti-CACNB4 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O00305
Species Reactivity
Human, Mouse, Rat, Hamster
Specificity
Detects ~50 kDa. No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide amino acids 458-474 of rat CavB4
Cellular Localization
Cytoplasm; Cell Membrane; Synapse
Certificate of Analysis
1 ug/ml was sufficient for detection of Cavbeta4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-CACNB4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CACNB4 antibody
This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized (1, 2).
Product Categories/Family for anti-CACNB4 antibody
Neuroscience; Ion Channels; Calcium Channels; Voltage-Gated Calcium Channels; Cancer; Cell Signaling; Protein Trafficking
Applications Tested/Suitable for anti-CACNB4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Antibody Microarray (AM)
Application Notes for anti-CACNB4 antibody
WB: 1:1000
IHC: 1:1000
ICC/IF: 1:100
Immunohistochemistry (IHC) of anti-CACNB4 antibody
Immunohistochemistry analysis using Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody, Clone S10-7 at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Western Blot (WB) of anti-CACNB4 antibody
Western Blot analysis of hamster T-CHO cell lysate showing detection of CACNB4 Calcium Channel protein using Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody, Clone S10-7 at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
NCBI/Uniprot data below describe general gene information for CACNB4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000717
[Other Products]
NCBI GenBank Nucleotide #
NP_000717.2
[Other Products]
UniProt Primary Accession #
O00305
[Other Products]
UniProt Related Accession #
O00305[Other Products]
NCBI Official Full Name
voltage-dependent L-type calcium channel subunit beta-4 isoform b
NCBI Official Synonym Full Names
calcium voltage-gated channel auxiliary subunit beta 4
NCBI Official Symbol
CACNB4 [Similar Products]
NCBI Official Synonym Symbols
EA5; EJM; CAB4; EIG9; EJM4; EJM6; CACNLB4
[Similar Products]
NCBI Protein Information
voltage-dependent L-type calcium channel subunit beta-4
UniProt Protein Name
Voltage-dependent L-type calcium channel subunit beta-4
UniProt Synonym Protein Names
Calcium channel voltage-dependent subunit beta 4
Protein Family
Voltage-dependent L-type calcium channel
UniProt Gene Name
CACNB4 [Similar Products]
UniProt Synonym Gene Names
CACNLB4; CAB4 [Similar Products]
UniProt Entry Name
CACB4_HUMAN
NCBI Summary for CACNB4
This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE), juvenile myoclonic epilepsy (JME), and episodic ataxia, type 5. [provided by RefSeq, Aug 2016]
Product References and Citations for anti-CACNB4 antibody
1. "Entrez Gene: CACNB4 calcium channel, voltage-dependent, beta 4 subunit". 2. Xie M., et al. (2007) J Cell Biol. 178(3): 489-502.
Research Articles on CACNB4
1. The nuclear targeting properties of the truncated beta(4b(1-481)) subunit in tsA-201 cells, skeletal myotubes, and in hippocampal neurons, were investigated.
Precautions
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Disclaimer
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