Thromboxane A2, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TX-A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS741624 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thromboxane A2 (TX-A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TX-A2. The ELISA analytical biochemical technique of the MBS741624 kit is based on TX-A2 antibody-TX-A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TX-A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TX-A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: TX-A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TX-A2 antibody and an TX-A2-HRP conjugate. The assay sample and buffer are incubated together with TX-A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TX-A2 concentration since TX-A2 from samples and TX-A2-HRP conjugate compete for the anti-TX-A2 antibody . Since the number of sites is limited, as more sites are occupied by TX-A2 from the sample, fewer sites are left to bind TX-A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TX-A2 concentration in each sample is interpolated from this standard curve.

Cardiovascular

44,205 Da

thromboxane A2 receptor

thromboxane A2 receptor; prostanoid TP receptor

Thromboxane A2 receptor

TXA2-R  [Similar Products]

TA2R_HUMAN

This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

TXA2-R: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C. Isoform 1 activates adenylyl cyclase. Isoform 2 inhibits adenylyl cyclase. Defects in TBXA2R are the cause of susceptibility to bleeding disorder platelet-type 13 (BDPLT13). BDPLT13 is a disorder characterized by reduced platelet aggregation and a tendency to mild mucocutaneous bleeding. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: integral to plasma membrane; plasma membrane; acrosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; thromboxane A2 receptor activity

Biological Process: response to drug; second-messenger-mediated signaling; platelet activation; response to lipopolysaccharide; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; positive regulation of vasoconstriction; positive regulation of smooth muscle contraction; blood coagulation; inflammatory response; positive regulation of blood pressure; response to nutrient; positive regulation of GTPase activity

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