Full Product Name
FBP1 siRNA (Mouse)
Product Synonym Names
FBP; FBP3; Fructose-1.6-bisphosphatase 1; FBPase 1; D-fructose-1.6-bisphosphate 1-phosphohydrolase 1; Fructose-1.6-bisphosphatase isozyme 3; FBPase 3; Liver FBPase
Product Gene Name
FBP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9QXD6
Specificity
FBP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FBP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FBP1 sirna
siRNA to inhibit FBP1 expression using RNA interference
Applications Tested/Suitable for FBP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FBP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062268.1
[Other Products]
NCBI GenBank Nucleotide #
NM_019395.3
[Other Products]
UniProt Primary Accession #
Q9QXD6
[Other Products]
UniProt Secondary Accession #
P97323; Q3UEH1[Other Products]
UniProt Related Accession #
Q9QXD6[Other Products]
Molecular Weight
36,912 Da
NCBI Official Full Name
fructose-1,6-bisphosphatase 1
NCBI Official Synonym Full Names
fructose bisphosphatase 1
NCBI Official Symbol
Fbp1 [Similar Products]
NCBI Official Synonym Symbols
Fbp2; Fbp3; Fbp-2
[Similar Products]
NCBI Protein Information
fructose-1,6-bisphosphatase 1
UniProt Protein Name
Fructose-1,6-bisphosphatase 1
UniProt Synonym Protein Names
D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; Fructose-1,6-bisphosphatase isozyme 3; FBPase 3; Liver FBPase
Protein Family
Fat-body protein
UniProt Gene Name
Fbp1 [Similar Products]
UniProt Synonym Gene Names
Fbp; Fbp3; FBPase 1; FBPase 3 [Similar Products]
UniProt Entry Name
F16P1_MOUSE
UniProt Comments for FBP1
FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
Protein type: Mitochondrial; Carbohydrate Metabolism - pentose phosphate pathway; Phosphatase (non-protein); EC 3.1.3.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose
Cellular Component: cytoplasm; cytosol
Molecular Function: monosaccharide binding; identical protein binding; hydrolase activity; metal ion binding; fructose-bisphosphatase activity; catalytic activity; AMP binding; phosphoric ester hydrolase activity
Biological Process: dephosphorylation; metabolic process; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; regulation of gluconeogenesis; negative regulation of cell growth; fructose 6-phosphate metabolic process; negative regulation of glycolysis; gluconeogenesis; protein homotetramerization
Research Articles on FBP1
1. The Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
