Fructose-1, 6-bisphosphatase 1 (FBP1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of FBP1. No significant cross-reactivity or interference between FBP1and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBP1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of FBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7227331 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fructose-1, 6-bisphosphatase 1 (FBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FBP1. The ELISA analytical biochemical technique of the MBS7227331 kit is based on FBP1 antibody-FBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses||This FBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBP1 antibody and an FBP1-HRP conjugate. The assay sample and buffer are incubated together with FBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theFBP1 concentration since FBP1 from samples and FBP1-HRP conjugate compete for the anti-FBP1 antibody . Since the number of sites is limited, as more sites are occupied by FBP1 from the sample, fewer sites are left to bind FBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBP1 concentration in each sample is interpolated from this standard curve.

36,842 Da

fructose-1,6-bisphosphatase 1

fructose-1,6-bisphosphatase 1; FBPase 1; liver FBPase; fructose-bisphosphatase 1; growth-inhibiting protein 17; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1

Fructose-1,6-bisphosphatase 1

FBP; FBPase 1  [Similar Products]

F16P1_HUMAN

Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008]

FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.

Protein type: EC 3.1.3.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; Mitochondrial

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: monosaccharide binding; identical protein binding; protein binding; metal ion binding; fructose-bisphosphatase activity; AMP binding

Biological Process: dephosphorylation; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; glucose metabolic process; regulation of gluconeogenesis; pathogenesis; negative regulation of cell growth; fructose 6-phosphate metabolic process; negative regulation of glycolysis; fructose metabolic process; gluconeogenesis; protein homotetramerization

Disease: Fructose-1,6-bisphosphatase Deficiency

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