1. Add sufficient collagen solution to coat dishes, plates or inserts. 1-2 ml of solution is sufficient to cover a 35 mm dish.
2. Incubate at room temperature in a
biological safety cabinet partially covered for 1 hour.
3. Carefully aspirate remaining solution. Rinse with proper volume of serum-free media to remove buffer solution.
4. Coated plates are best used immediately but can be air-dried and stored.
5. Coated plates that have not been cultured can be dried and stored at 2 -8 degree C and are useful up to 1 week under sterile conditions
Wells: 6; Area(cm2): 4.67; Coating Volume (mL): 0.6; Wash Volume (mL): 1
Wells: 12; Area(cm2): 1.12; Coating Volume (mL): 0.25; Wash Volume (mL): 0.4
Wells: 24; Area(cm2): 0.33; Coating Volume (mL): 0.05; Wash Volume (mL): 0.1
Wells: 75mm insert; Area(cm2): 44; Coating Volume (mL): 5; Wash Volume (mL): 8
Wells: 96; Area(cm2): 0.143; Coating Volume (mL): 0.025; Wash Volume (mL): 0.05
