Adrenomedullin 2 (ADM2), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.11 (50481556..50486437). Location: 22q13.33

This assay has high sensitivity and excellent specificity for detection of ADM2. No significant cross-reactivity or interference between ADM2 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2023596 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adrenomedullin 2 (ADM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADM2. The ELISA analytical biochemical technique of the MBS2023596 kit is based on ADM2 antibody-ADM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

15,865 Da

adrenomedullin 2

ADM2; intermedin

ADM2

AM2; AM2; IMDL; IMDS  [Similar Products]

ADM2_HUMAN

This gene encodes a member of the calcitonin gene-related peptide (CGRP)/calcitonin family of hormones that play a role in the regulation of cardiovascular homeostasis, prolactin release, anti-diuresis, anti-natriuresis, and regulation of food and water intake. The encoded protein is proteolytically processed to generate one or more biologically active peptides. [provided by RefSeq, Jul 2015]

ADM2: IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway. Belongs to the adrenomedullin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: extracellular region

Molecular Function: hormone activity; protein complex binding

Biological Process: positive regulation of angiogenesis; digestion; feeding behavior; negative regulation of blood pressure; angiogenesis; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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