RAC2 Human, Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (37621301..37640339, complement). Location: 22q13.1

Affinity Purified
~95% by SDS-PAGE Purified by immunoaffinity chromatography.

Supplied as liquid. In 20 mM Tris-HCl buffer, pH 8.0, 20% Glycerol 2mM EDTA, 2mM DTT, 0.1 M NaCl.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of RAC2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rac2 is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. Rac proteins play important roles in multiple cellular events, including actin cytoskeletal organization, cell proliferation and survival, cell cycle progression, and gene transcription regulation. Recombinant human RAC2, expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Biology; MB-Protein Kinases

Suitable for use in ELISA and Western Blot.

23.3kD[Similar Products]

ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

ras-related C3 botulinum toxin substrate 2; small G protein; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)

Ras-related C3 botulinum toxin substrate 2

RAC2_HUMAN

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]

RAC2: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Interacts with DOCK2, which may activate it. Hematopoietic specific. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: focal adhesion; lamellipodium; plasma membrane; nuclear envelope; actin filament; cytosol

Molecular Function: GTPase activity; GTP binding

Biological Process: platelet activation; axon guidance; metabolic process; actin filament organization; cell projection biogenesis; signal transduction; G-protein coupled receptor protein signaling pathway; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of cell proliferation; blood coagulation; regulation of hydrogen peroxide metabolic process; bone resorption

Disease: Neutrophil Immunodeficiency Syndrome

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.