ZEB2, Polyclonal Antibody

SIP-1; SIP1; Smad interacting-protein 1; Smad-interacting 1; SMADIP1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total ZEB2 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-ZEB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.Cacheux V., Hum. Mol. Genet. 10:1503-1510(2001).Wakamatsu N., Nat. Genet. 27:369-370(2001).Nagase T., DNA Res. 5:31-39(1998).

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HepG2 cells, using ZEB2 antibody.

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZEB2 antibody.

133,806 Da

zinc finger E-box binding homeobox 2

zinc finger E-box-binding homeobox 2

Zinc finger E-box-binding homeobox 2

KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1  [Similar Products]

ZEB2_HUMAN

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]

ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: Transcription, coactivator/corepressor; DNA-binding; Motility/polarity/chemotaxis; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: nucleus

Molecular Function: DNA binding; phosphatase regulator activity; metal ion binding

Biological Process: nervous system development; positive regulation of JNK activity; somitogenesis; transcription, DNA-dependent; cell proliferation in forebrain; hippocampus development; pigmentation during development; positive regulation of melanin biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of melanocyte differentiation; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; neural crest cell migration

Disease: Mowat-wilson Syndrome

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