ZEB2, Polyclonal Antibody

ZEB2; zinc finger E-box binding homeobox 2; FLJ42816; KIAA0569; SIP-1; SIP1; SMADIP1; ZFHX1B; OTTHUMP00000204540; OTTHUMP00000204541; Smad-interacting protein 1; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; zinc finger homeobox protein 1; ZEB2 antibody; zinc finger E-box binding homeobox 2 antibody; FLJ42816 antibody; KIAA0569 antibody; SIP-1 antibody; SIP1 antibody; SMADIP1 antibody; ZFHX1B antibody; OTTHUMP00000204540 antibody; Smad-interacting protein 1 antibody; zinc finger E-box-binding homeobox 2 antibody; zinc finger homeobox 1b antibody; zinc finger homeobox protein 1 antibody; ZEB2 (aa545-558)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-ZEB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 100+38kDa bands observed in Human Liver lysates (calculated MW of 136kDa according to NP_055610.1). Recommended concentration: 0.2-0.5ug/ml. We believe the two observed bands represent two breakdown fragments.

(0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

133,806 Da

zinc finger E-box binding homeobox 2

zinc finger E-box-binding homeobox 2

Zinc finger E-box-binding homeobox 2

KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1  [Similar Products]

ZEB2_HUMAN

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]

ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; phosphatase regulator activity; protein binding

Biological Process: cell proliferation in forebrain; collateral sprouting; corpus callosum morphogenesis; corticospinal tract morphogenesis; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; nervous system development; neural crest cell migration; neural tube closure; pigmentation during development; positive regulation of axonogenesis; positive regulation of JNK activity; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; somitogenesis; transcription from RNA polymerase II promoter

Disease: Mowat-wilson Syndrome

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