ALKB Alkylation Repair Homolog 3, Recombinant Protein

E Coli

Greater than 95.0% as determined by SDS-PAGE.

The ALKBH3 solution 1mg/ml contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Sterile Filtered colorless solution.

Small volumes of ALKBH3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: ALKBH3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 325 amino acids (1-286 a.a.) and having a molecular mass of 37.9kDa. The ALKBH3 is purified by proprietary chromatographic techniques.

Introduction: ALKBH3 belongs to the ALKB family of proteins and acts as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a broad array of tissues and localizes to the cytoplasm and the nucleus.

ENZYMES; Enzymes

19,310 Da

alkB, alkylation repair homolog 3 (E. coli)

alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; alkylated DNA repair protein alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

ABH3; DEPC1  [Similar Products]

ALKB3_HUMAN

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]

ALKBH3: Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; DNA repair, damage; Hydrolase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; L-ascorbic acid binding; ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity

Biological Process: cell proliferation; DNA dealkylation; DNA repair

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.