CLK2, Polyclonal Antibody

Dual specificity protein kinase CLK2; CDC-like kinase 2; CLK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the Central region of human CLK2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CLK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the CLK family of dual
specificity protein kinases. CLK family members have been shown to
interact with, and phosphorylate, serine- and arginine-rich (SR)
proteins of the spliceosomal complex, which is a part of the
regulatory mechanism that enables the SR proteins to control RNA
splicing. Note that this gene is distinct from TELO2 gene
(GeneID:9894), which shares CLK2 and hCLK2 symbol aliases in common
with this gene, but encodes a protein that is involved in telomere
length regulation.

Cell Biology; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

CLK2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CLK2 antibody detected the CLK2 protein (arrow).

60090

CDC-like kinase 2

dual specificity protein kinase CLK2

Dual specificity protein kinase CLK2

CLK2_HUMAN

This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Kinase, protein; Protein kinase, CMGC; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: response to retinoic acid; peptidyl-tyrosine phosphorylation; regulation of RNA splicing; negative regulation of gluconeogenesis; protein amino acid autophosphorylation; response to ionizing radiation; protein amino acid phosphorylation

Nam, S.Y., et al. J. Biol. Chem. 285(41):31157-31163(2010)
Eisenreich, A., et al. Circ. Res. 104(5):589-599(2009)
Rendtlew Danielsen, J.M., et al. J. Biol. Chem. 284(7):4140-4147(2009)
Castle, J.C., et al. Nat. Genet. 40(12):1416-1425(2008)
Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004)

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