Double-stranded RNA-specific adenosine deaminase, ELISA Kit

Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP; ADAR; ADAR1; DSRAD;

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of ADAR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2887760 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Double-stranded RNA-specific adenosine deaminase (ADAR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAR. The ELISA analytical biochemical technique of the MBS2887760 kit is based on ADAR antibody-ADAR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

102,580 Da

adenosine deaminase, RNA-specific

double-stranded RNA-specific adenosine deaminase

Double-stranded RNA-specific adenosine deaminase

DRADA  [Similar Products]

DSRAD_MOUSE

ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Nucleolus; RNA-binding; EC 3.5.4.37; RNA processing; Hydrolase

Cellular Component: cytoplasm; membrane; nuclear speck; nucleolus; nucleoplasm; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; left-handed Z-DNA binding; protein binding

Biological Process: adenosine to inosine editing; base conversion or substitution editing; defense response to virus; erythrocyte differentiation; hemopoietic progenitor cell differentiation; in utero embryonic development; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; negative regulation of apoptosis; negative regulation of viral genome replication; osteoblast differentiation; positive regulation of viral genome replication; pre-microRNA processing; protein export from nucleus; protein import into nucleus; response to virus; somatic diversification of immune receptors via somatic mutation

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