Product Name
IFIH1, siRNA
Full Product Name
IFIH1 siRNA (Mouse)
Product Synonym Names
Interferon-induced helicase C domain-containing protein 1; Helicase with 2 CARD domains; Helicard; Interferon induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; RIG-I-like receptor 2; RLR-2
Product Gene Name
IFIH1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8R5F7
Specificity
IFIH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IFIH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IFIH1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IFIH1 sirna
siRNA to inhibit IFIH1 expression using RNA interference
Applications Tested/Suitable for IFIH1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IFIH1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001157949.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001164477.1
[Other Products]
UniProt Primary Accession #
Q8R5F7
[Other Products]
UniProt Secondary Accession #
Q3U6S2; Q68EM4; Q8BYC9; Q8BZ01; Q8K5C7; Q8R144; Q8VE79; Q99KS4; Q9D2Z5; A2AUY7[Other Products]
UniProt Related Accession #
Q8R5F7[Other Products]
Molecular Weight
110,842 Da
NCBI Official Full Name
interferon-induced helicase C domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
interferon induced with helicase C domain 1
NCBI Official Symbol
Ifih1 [Similar Products]
NCBI Official Synonym Symbols
Hlcd; MDA5; RLR-2; Helicard; 9130009C22Rik
[Similar Products]
NCBI Protein Information
interferon-induced helicase C domain-containing protein 1
UniProt Protein Name
Interferon-induced helicase C domain-containing protein 1
UniProt Synonym Protein Names
Helicase with 2 CARD domains; Helicard; Interferon induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; RIG-I-like receptor 2; RLR-2
Protein Family
Interferon-induced helicase C domain-containing protein
UniProt Gene Name
Ifih1 [Similar Products]
UniProt Synonym Gene Names
Helicard; MDA-5; RLR-2 [Similar Products]
UniProt Entry Name
IFIH1_MOUSE
UniProt Comments for IFIH1
IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Helicase; Apoptosis; EC 3.6.4.13
Cellular Component: cytoplasm; nucleus
Molecular Function: ribonucleoprotein binding; protein binding; single-stranded RNA binding; hydrolase activity, acting on acid anhydrides; DNA binding; hydrolase activity; zinc ion binding; RNA binding; metal ion binding; double-stranded RNA binding; nucleotide binding; helicase activity; ATP binding
Biological Process: regulation of apoptosis; protein sumoylation; immune system process; response to virus; positive regulation of interferon-beta production; innate immune response; defense response to virus; positive regulation of interferon-alpha production
Research Articles on IFIH1
1. IFIH1 heterozygous mice have a regulatory rather than effector T-cell response at the site of autoimmunity, supporting IFIH1 expression as an essential regulator of the diabetogenic T-cell response.
Precautions
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Disclaimer
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