Forkhead box protein L2 (FOXL2), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

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Small volumes of FOXL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7208699 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Forkhead box protein L2 (FOXL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOXL2. The ELISA analytical biochemical technique of the MBS7208699 kit is based on FOXL2 antibody-FOXL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOXL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOXL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

38,772 Da

forkhead box L2

forkhead box protein L2; forkhead transcription factor FOXL2

Forkhead box protein L2

FOXL2_HUMAN

This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jun 2009]

FOXL2: Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans- differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES); also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II. Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; caspase regulator activity; estrogen receptor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; embryonic eye morphogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; female somatic sex determination; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; ovarian follicle development; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; cell differentiation; menstruation; extraocular skeletal muscle development; negative regulation of transcription, DNA-dependent; granulosa cell differentiation

Disease: Blepharophimosis, Ptosis, And Epicanthus Inversus; Premature Ovarian Failure 3

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