BMP-6, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinant Monkey BMP-6. No significant cross-reactivity or interference between Monkey BMP-6 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BMP-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2514695 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP-6, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP-6. The ELISA analytical biochemical technique of the MBS2514695 kit is based on BMP-6 antibody-BMP-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey BMP-6 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BMP-6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BMP-6. You can calculate the concentration of BMP-6 in the samples by comparing the OD of the samples to the standard curve.

57,226 Da

bone morphogenetic protein 6

bone morphogenetic protein 6; BMP-6; VG-1-R; VG-1-related protein; VGR-1; Vg1-related sequence; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine

Bone morphogenetic protein 6

VGR; BMP-6; VG-1-R; VGR-1  [Similar Products]

BMP6_HUMAN

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]

BMP6: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24-p23

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; protein heterodimerization activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: cellular iron ion homeostasis; response to glucocorticoid stimulus; regulation of apoptosis; BMP signaling pathway; response to magnesium ion; positive regulation of endothelial cell differentiation; positive regulation of aldosterone biosynthetic process; male genitalia development; kidney development; inflammatory response; skeletal development; response to iron ion; endochondral ossification; response to retinoic acid; positive regulation of bone mineralization; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; positive regulation of protein secretion; eye development; cartilage development; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of endothelial cell proliferation; response to activity; positive regulation of neuron differentiation; positive regulation of epithelial cell proliferation; growth

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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