GABA(A) Receptor Beta3, Monoclonal Antibody

Gabrb3; GABA(A) receptor subunit beta3

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

S87-25

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Gabrb3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects ~55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.

Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).

Ion Channels, Neuroscience

Western Blot (WB), Immunocytochemistry (ICC)

1ug/mL (WB), 0.1-1ug/mL (IHC/ICC), 1-10ug/mL (IF)

Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

54,166 Da[Similar Products]

gamma-aminobutyric acid (GABA) A receptor, subunit beta 3

gamma-aminobutyric acid receptor subunit beta-3; cleft palate 1; GABA(A) receptor subunit beta-3; gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3

Gamma-aminobutyric acid receptor subunit beta-3

Gabrb-3  [Similar Products]

GBRB3_MOUSE

GABRB3: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRB3 are associated with chronic insomnia, a condition of inability to initiate or maintain sleep. This may occur as a primary disorder or in association with another medical or psychiatric condition. Defects in GABRB3 are the cause of childhood absence epilepsy type 5 (ECA5). ECA5 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into *****hood. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, integral; Channel, chloride; Transporter, ion channel; Membrane protein, multi-pass; Transporter

Cellular Component: postsynaptic membrane; membrane; integral to plasma membrane; integral to membrane; plasma membrane; terminal button; synapse; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; ion channel activity; extracellular ligand-gated ion channel activity; GABA-gated chloride ion channel activity

Biological Process: sensory perception of sound; transport; inner ear receptor cell development; regulation of neuron apoptosis; synaptic transmission, GABAergic; neuron development; ion transport; chloride transport; negative regulation of neuron apoptosis; palate development; signal transduction; gamma-aminobutyric acid signaling pathway

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 4. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.

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