GLIS family zinc finger 2, ELISA Kit

Mouse Zinc finger protein GLIS2 (GLIS2) ELISA kit; FLJ38247; NPHP7; Kruppel-like zinc finger protein GLIS2; nephrocystin-7; GLIS family zinc finger 2

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of GLIS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9331322 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GLIS family zinc finger 2 (GLIS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLIS2. The ELISA analytical biochemical technique of the MBS9331322 kit is based on GLIS2 antibody-GLIS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLIS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLIS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

55,842 Da

GLIS family zinc finger 2

zinc finger protein GLIS2; GLI-similar 2; zinc finger protein GLI5; GLI-Kruppel family member GLI5; neuronal Krueppel-like protein; Kruppel-like factor 16 (neuronal)

Zinc finger protein GLIS2

Gli5; Nkl  [Similar Products]

GLIS2_MOUSE

GLIS2: Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation. Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7). NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Cellular Component: transcription factor complex; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transcription, DNA-dependent

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