Complement C1q subcomponent subunit A, ELISA Kit

C1qa (Complement C1q subcomponent subunit A)/Complement Component 1q Subcomponent; A Chain

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of C1qa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763220 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement C1q subcomponent subunit A (C1qa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C1qa. The ELISA analytical biochemical technique of the MBS763220 kit is based on C1qa antibody-C1qa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C1qa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C1qa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse C1qa antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse C1qa antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse C1qa amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse C1qa can be calculated.

26,017 Da

complement component 1, q subcomponent, A chain

complement C1q subcomponent subunit A

Complement C1q subcomponent subunit A

C1QA_HUMAN

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq, Jul 2008]

C1QA iso2: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2 )-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: collagen; complement component C1 complex; extracellular region

Molecular Function: protein binding

Biological Process: cell-cell signaling; complement activation; complement activation, classical pathway; innate immune response; response to iron ion

Disease: C1q Deficiency

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