DIAPH3, Polyclonal Antibody

Protein diaphanous homolog 3; Diaphanous-related formin-3; DRF3; MDia2; DIAPH3; DIAP3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-DIAPH3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).

Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

DIAPH3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DIAPH3 antibody detected the DIAPH3 protein (arrow).

136926

diaphanous-related formin 3

protein diaphanous homolog 3

Protein diaphanous homolog 3

DIAP3; DRF3  [Similar Products]

DIAP3_HUMAN

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Diaphanous-3: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Defects in DIAPH3 are the cause of auditory neuropathy, autosomal dominant, type 1 (AUNA1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. A disease- causing mutation in the conserved 5'-UTR leads to increased protein expression (PubMed:20624953). Belongs to the formin homology family. Diaphanous subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q21.2

Cellular Component: cytosol; nucleus

Molecular Function: Rho GTPase binding; actin binding

Biological Process: spermatogenesis; actin cytoskeleton organization and biogenesis

Disease: Auditory Neuropathy, Autosomal Dominant, 1

DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009)
Block, J., et al. J Microsc 231(3):506-517(2008)
Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008)
Yang, C., et al. PLoS Biol. 5 (11), E317 (2007) :
Gupton, S.L., et al. J. Cell. Sci. 120 (PT 19), 3475-3487 (2007) :

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